Here i wirte the coding for removing the selcted item from the listbox when we click a button.
In case if this is not u r expectation then send a mail to me....


Private Sub Button1_Click(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles Button1.Click
Dim n As Integer
n = ListBox1.Items.Count
MsgBox(n)
ListBox1.Items.Remove(ListBox1.SelectedItem)
End Sub

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